Fitness & Nutrition News

Fatty liver disease often builds quietly. There are no loud symptoms, no immediate warning signs, and yet the liver slowly fills with fat. For many, the diagnosis feels alarming. But there is one reassuring truth, this is one of the few conditions where the body can genuinely heal itself if given the right support.The real question is not whether it can reverse without medication. It is whether daily habits are aligned with what the liver actually needs.

Discover more about the pathways leading to adverse maternal and neonatal outcomes in women who experience interpersonal racial discrimination.

Osteopathic manipulative treatment may offer a new way to support pulmonary function in asthma. Read more.

DIETS, long-term, tend not to have a benefit. Once you come off them, your weight rebounds. And that’s because diets are always about...

Toxoplasmosis is a major foodborne zoonosis causing high global disease burden and economic losses in sheep and goat industries. The gold standard for detecting viable Toxoplasma gondii is the mouse bioassay, which involves inoculating tissues from infected animals into laboratory mice. Here, we describe a faster, cost-effective, and ethical alternative method to assess T. gondii presence and viability. The procedure is based on reverse transcription quantitative PCR (RT-qPCR) targeting messenger RNA transcripts of genes highly expressed during infection, including the bradyzoite-specific gene bag1, indicative of chronic infection and gra1, which detects both acute and chronic infections due to high transcription in tachyzoites and bradyzoites. This RT-qPCR assay was evaluated alongside the mouse bioassay and two molecular methods, 529 bp-specific qPCR and nested ITS-1 PCR, using tissues from experimentally infected piglets and sheep. Cohen’s kappa coefficient showed moderate agreement between gra1–bag1 RT-qPCR and the mouse bioassay (κ = 0.557), comparable to 529-qPCR (κ = 0.556). The assay detected gra1 and bag1 transcripts in all bioassay-positive samples, demonstrating high predictive value for viable parasites. The gra1–bag1 RT-qPCR provides a reliable prescreening tool for predicting parasite viability in tissues, supporting ethical research by reducing reliance on the mouse bioassay.

Is it possible that your diet can protect you from getting Alzheimer's?

Tarina "Richie" Green details how her mommy makeover impacted her most recent pregnancy

Stephanie James and Mario Ciccarello went out to dinner to mark Valentine's Day before tragedy struck

As the star heads toward 60, she's revealed the lifestyle changes that have helped her feel her best

Women who are pregnant or planning to conceive are being urged to limit their exposure to everyday chemicals that may interfere with hormones, amid growing concern about their potential long-term effects on both mother and child.